- Take the solvent vial by its cap and shake well to make sure all liquid in the vial has dropped on the bottom. Open the solvent vial and put it in a vertical position.
- To collect a sample from the surface of the vagina use the sterile polyester swab provided. Remove the sterile swab from its package following instructions on the package. The polyester tip should not touch anything prior to its insertion into vagina. Hold the swab in the middle of the stick and, while the patient is lying on her back, carefully insert the polyester tip of the swab into the vagina until the fingers contact the skin no more than 2-3 inches (5-7 cm) deep. Withdraw the swab from the vagina after 1 minute.
- Place the polyester tip into the vial and rinse the swab in the solvent by rotating for one minute.
- Remove and dispose of the swab.
- Tear open the foil pouch at the tear notches and remove the AmniSure® test strip.
- Dip the white end of the test strip (marked with arrows) into the vial with solvent. Strong leakage of amniotic fluid may make the results visible early (within 5 minutes), while a very small leak will take the full 10 minutes.
- Remove the test strip if two stripes are clearly visible in the vial or after 10 minutes sharp. Read the results by placing the test on a clean, dry, flat surface. Do not read or interpret the results after 15 minutes have passed since dipping the test strip into the vial.
- The darkness of the stripes may vary. The test is valid even if the stripes are faint or uneven. Do not try to interpret the test result based on the darkness of the stripes
- If only a control line is visible, the test result is negative. If both control and test line are visible, the test result is positive. If no lines are visible, the test result is invalid.
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